Boil Purification of Genomic DNA

 

Protocol:

  1. Add 50 µl of TE buffer to a PCR tube or Microcentrifuge tube

  2. Using sterile technique, pick up a colony from your plate and shake the loop around in the 50 µl of TE buffer to detach some cells.

  3. Heat the sample to 95°C for 15 minutes in a heat block or thermal cycler.

    • Optional: Centrifuge at max speed for 5 minutes to pellet the cells. Most of the genomic DNA should remain within the liquid supernatant. Gently remove it and add it to another tube, using a pipette and careful not to disturb the pellet.

Using the purified Genomic DNA for PCR:

  1. Add 1-2 µl of your sample to your PCR master mix.

    • Note: Do not add more sample, or the EDTA in the TE buffer will inhibit the polymerase activity

Protocol: Polymerase Chain Reaction


Visualising the purified Genomic DNA on a gel:

  1. Mix 3-7 µl of your sample with 3 µl of 6 x purple loading dye.

Protocol: Agarose Gel Electrophoresis

Acknowledgements: