Buffer P2 (Lysis Buffer)

Final Buffer Concentration: 

• 200 mM NaOH 

• 1 % SDS

Equipment & Consumables

• PPE: Gloves, Lab Coat and Goggles (at least!)

• Sterile Workspace

• Sterile Micropipettes and Tips

• Pipetteboi and tips

• Weighing Scale (accurate to to 0.01g)

  • Weigh boats (or folded paper)

• Filters & Syringes OR Autoclave

• Optional: Heated/Stirring Platform

• pH Meter (recently calibrated)

• Graduated Beaker

• Measuring Cylinder

• 4°C Refrigerator

• ddH20 (MilliQ or RO)

• NaOH - Sodium Hydroxide

• SDS - Sodium Dodecyl sulfate

Protocol:

For Mixing 200 ml of Buffer:

1. Add 1.60 g of NaOH to 20 ml dH2O.

2. Add 2 g of SDS and allow everything to dissolve.

3. Make up to 200 ml.

See video for 100 ml and 1 L recipes, safety instructions and much, much more;

Video: